ZymoPURE II Plasmid Maxiprep Kit by Zymo Research – 10 Ultra Pure Prep

Description

Product Overview

The ZymoPURE II Plasmid Maxiprep Kit (model D4202) is engineered to deliver up to 1.2 mg of ultra‑pure, endotoxin‑free plasmid DNA per preparation, making it one of the most efficient solutions for high‑yield plasmid purification. Each kit contains all reagents required for ten independent maxiprep extractions, packaged in a compact box measuring 12.9 x 8.2 x 8 inches and weighing approximately 4 pounds, which simplifies storage and handling in busy laboratory environments. The kit utilizes a silica‑based spin column technology that combines rapid binding, thorough washing, and gentle elution steps, ensuring that the final DNA is free from protein contaminants, RNA, and endotoxin residues. The protocol is optimized for both high‑copy and low‑copy plasmids, providing consistent yields across a wide range of bacterial strains. All components are manufactured in the United States under strict quality‑control standards, and the kit is compatible with standard microcentrifuges and benchtop equipment commonly found in molecular biology labs.

Usage

This kit is designed for researchers who require reliable, high‑quality plasmid DNA for downstream applications such as restriction enzyme digestion, ligation, sequencing, transfection, and CRISPR‑Cas9 genome editing. It performs exceptionally well in academic research settings, biotech startups, and large‑scale industrial laboratories where reproducibility and purity are critical. The straightforward protocol, which can be completed in under 30 minutes per prep, reduces hands‑on time and allows scientists to focus on experimental design rather than purification logistics. The kit is also suitable for teaching laboratories, providing students with a clear, step‑by‑step workflow that demonstrates the principles of nucleic acid purification. Because the reagents are endotoxin‑free, the DNA is ideal for sensitive cell‑based assays, including mammalian cell transfection and viral vector production, where even trace amounts of endotoxin can affect cell viability and experimental outcomes.

Why Choose Us

Choosing the ZymoPURE II Plasmid Maxiprep Kit means selecting a product backed by over a decade of expertise in nucleic acid purification. Zymo Research is a trusted name in the life‑science community, known for delivering reagents that meet rigorous performance standards. The kit’s components are produced in a GMP‑compliant facility in the United States, ensuring batch‑to‑batch consistency and traceability. Each lot undergoes extensive validation, including endotoxin testing, to guarantee that the DNA meets the stringent requirements of sensitive downstream applications. In addition, Zymo Research provides comprehensive technical support, with a dedicated customer service team ready to assist with protocol optimization, troubleshooting, and experimental design advice. The company also offers a satisfaction guarantee, reflecting confidence in the product’s ability to meet or exceed user expectations. By investing in this kit, laboratories benefit from reduced variability, higher success rates in cloning and expression experiments, and a streamlined workflow that saves both time and resources.

Key Features

• High‑yield purification delivering up to 1.2 mg of ultra‑pure plasmid DNA per prep.
• Endotoxin‑free reagents ensure compatibility with sensitive cell‑based assays.
• Simple 10‑step protocol completed in under 30 minutes, reducing hands‑on time.
• Compact, lightweight packaging for easy storage and handling in any lab environment.
• Dedicated Zymo Research technical support and satisfaction guarantee for reliable after‑sales service.

FAQ

What is the maximum amount of plasmid DNA I can obtain from a single prep?

The kit is optimized to yield up to 1.2 mg of high‑quality plasmid DNA per maxiprep, depending on plasmid copy number and bacterial growth conditions.

Is the DNA obtained endotoxin‑free?

Yes, the purification process removes endotoxin contaminants, making the DNA suitable for transfection and other applications where endotoxin sensitivity is a concern.

Can I use this kit for low‑copy plasmids?

Absolutely. The protocol is designed to work efficiently with both high‑copy and low‑copy plasmids, providing consistent yields across different plasmid types.

What equipment do I need to run the kit?

The kit requires a standard benchtop microcentrifuge capable of 13,000 × g, a heat block or water bath for incubation steps, and basic laboratory consumables such as pipettes and sterile tubes.

How many preps does one kit provide?

Each ZymoPURE II Plasmid Maxiprep Kit contains reagents for ten independent maxiprep extractions, allowing you to complete multiple experiments without restocking.