Description
Product Overview
The Zymo Research RNA Clean & Concentrator-5 Kit (model R1013) is engineered to provide rapid, high‑yield RNA purification for a broad spectrum of research applications. Combining a silica‑based spin column with an on‑column DNase treatment, the kit eliminates DNA contamination and removes inhibitors in just five minutes. Users benefit from a recovery rate exceeding 90% and the ability to elute RNA in volumes as low as 6 µl, preserving precious material for downstream workflows. The kit includes all necessary reagents for up to five preps, each pre‑filled with optimized buffers that maintain RNA integrity throughout the process. Package dimensions of 6.5 × 5.5 × 4.5 inches and a lightweight design (11.99 oz) make storage and handling straightforward in busy laboratory environments.
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Usage
This kit is ideal for laboratories performing RT‑qPCR, next‑generation sequencing (NGS), microarray hybridization, and other RNA‑dependent assays. The streamlined protocol requires minimal hands‑on time, allowing researchers to process multiple samples efficiently without sacrificing quality. Because the DNase step is integrated directly on the column, users avoid additional purification steps that could lead to sample loss. The kit performs reliably with a variety of sample types, including cultured cells, tissue homogenates, and body fluids, making it a versatile choice for both basic and translational research projects.
Why Choose Us
Zymo Research has a long‑standing reputation for delivering high‑performance nucleic acid purification solutions. The RNA Clean & Concentrator-5 Kit reflects this commitment through rigorous quality control, batch‑to‑batch consistency, and a design that prioritizes user convenience. Each component is manufactured under ISO‑9001 standards, and the kit is validated for reproducible RNA yields across a range of input amounts. In addition, Zymo offers responsive technical support and detailed protocol documentation, ensuring that users can quickly troubleshoot any issues and achieve optimal results.
Key Features
- Rapid five‑minute contaminant removal saves valuable bench time
- Integrated DNase treatment eliminates genomic DNA without extra steps
- Recover more than 90% of input RNA, preserving low‑abundance transcripts
- Elute in as little as 6 µl, concentrating samples for sensitive downstream assays
- Dedicated Zymo technical support provides guidance and troubleshooting
FAQ
Can this kit be used with low‑input RNA samples?
Yes, the kit’s ability to elute in volumes as low as 6 µl makes it suitable for low‑input samples, ensuring sufficient concentration for sensitive applications such as single‑cell RNA‑seq.
Is the DNase treatment compatible with downstream enzymatic reactions?
The on‑column DNase is fully inactivated during the wash steps, leaving no residual activity that would interfere with reverse transcription or PCR reactions.
What is the maximum sample volume that can be processed?
The protocol accommodates up to 500 µl of lysate per column, allowing flexibility for a wide range of starting material quantities.
How does the kit perform with tissue homogenates?
When combined with a suitable lysis buffer, the kit efficiently purifies RNA from tissue homogenates, removing proteins and other inhibitors that could affect downstream enzymatic reactions.
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